yana-notes

AMPK

2022-04-19: reference:

AMPK (AMP-activated protein kinase) #

  • ATP, ADP, and AMP all bind competitively to the same site. AMPK essentially detects the ratio of 1&2:3phosphate.

    • ATP allows, and ADP/AMP blocks phosphatases (PP2A, PP2C, pPM1E). AMP is also an allosteric modulator.
  • Phosphorylated (this means activation) by things like CAMKK2, LKB1, TAK1.

  • Insulin inhibits it by subsequent AKT and GSK-3β phosphorylation - both needed.

    • Can also be inhibited by Leptin, PKA, and PKC. S6K, and Protein Kinase D inhibit the β domain of the α subunit.
  • Its substrates are pretty random: acetyl-CoA carboxylase 1, SREBP1.

  • Phosphorylates Phosphofructokinase-2 (not 1 as in traditional glycolysis).

  • It stimulates things like hepatic Fatty Acid Oxidation, ketogenesis, inhibition of adipocyte Lipogenesis and Lipolysis, inhibits Gluconeogenesis quite directly

  • Upregulates PPARα, δ, cytochrome C.

  • Thyroid treatment increases AMPK activity/number in skeletal muscle.