Ser831

2022-05-07: reference:

GluR1 Ser831 #

This name might be sus at some point, but I’ll keep it. It’s the only result if you search online for Ser831.

• Phosphorylated by Protein Kinase C or CAMK II.
  • Thus CaMK II acts here in contrast to Ser-567 where it mediates LTD.
• Increases channel conductance (independent of agonist efficacy) and opening probability/frequency, decreasing activation energy for the intrasubunit conformational change. Also mediates receptor trafficking.

• Insulin induces phosphorylation of the AMPA receptor subunit GluR1, reversed by ZIP, and over-expression of Protein Kinase M zeta, reversed by amyloid beta

• Mechanism of CAMKII regulation of AMPA receptor gating

  • Increase in unitary conductance, γMEAN, by CaMKII in native receptors reflects phosphorylation of GluA1-Ser831, and is not an indirect result of phosphorylation of other GluA1 residues or associated regulatory proteins
  • Co-expression of TARPs is required for this increase in conductance.
    • Coexpression of stargazin increased γMEAN of both homo- and heteromeric receptors
      • CaMKII on recombinant AMPA receptors in the absence of TARPs showed that CaMKII can increase γMEAN in homomeric GluA1 but not GluA1/GluA2 heteromeric receptors.
        • Not sure what this implies, since Stargazin binds to both.
  • CAMK II enhances the coupling efficiency between agonist binding and channel gating
  • Does not appear to increase synaptic localization of GluA1-containing receptors.

• The CaMKII/NMDAR complex as a molecular memory